                         SEQUENCE LISTING



<110>  Novozymes A/S

      Andersen, Carsten 



<120>  Alpha-amylase variant with altered properties



<130>  10115.204-WO



<160>  17    



<170>  PatentIn version 3.1



<210>  1

<211>  1650

<212>  DNA

<213>  Bacillus sp.



<220>

<221>  CDS

<222>  (65)..(1567)

<223>  



<220>

<221>  mat_peptide

<222>  (128)..()

<223>  



<220>

<221>  sig_peptide

<222>  (65)..(128)

<223>  



<400>  1
cttgaatcat tatttaaagc tggttatgat atatgtaagc gttatcatta aaaggaggta     60

tttg atg aaa aga tgg gta gta gca atg ctg gca gtg tta ttt tta ttt     109
     Met Lys Arg Trp Val Val Ala Met Leu Ala Val Leu Phe Leu Phe        
         -20                 -15                 -10                    

cct tcg gta gta gtt gca gat ggc ttg aat gga acg atg atg cag tat      157
Pro Ser Val Val Val Ala Asp Gly Leu Asn Gly Thr Met Met Gln Tyr         
    -5              -1  1               5                   10          

tat gag tgg cat cta gag aat gat ggg caa cac tgg aat cgg ttg cat      205
Tyr Glu Trp His Leu Glu Asn Asp Gly Gln His Trp Asn Arg Leu His         
                15                  20                  25              

gat gat gcc gaa gct tta agt aat gcg ggt att aca gct att tgg ata      253
Asp Asp Ala Glu Ala Leu Ser Asn Ala Gly Ile Thr Ala Ile Trp Ile         
            30                  35                  40                  

ccc cca gcc tac aaa gga aat agt cag gct gat gtt ggg tat ggt gca      301
Pro Pro Ala Tyr Lys Gly Asn Ser Gln Ala Asp Val Gly Tyr Gly Ala         
        45                  50                  55                      

tac gac ctt tat gat tta ggg gag ttt aat caa aaa ggt acc gtt cga      349
Tyr Asp Leu Tyr Asp Leu Gly Glu Phe Asn Gln Lys Gly Thr Val Arg         
    60                  65                  70                          

acg aaa tac ggg aca aag gct cag ctt gag cga gct ata ggg tcc cta      397
Thr Lys Tyr Gly Thr Lys Ala Gln Leu Glu Arg Ala Ile Gly Ser Leu         
75                  80                  85                  90          

aag tcg aat gat atc aat gtt tat ggg gat gtc gta atg aat cat aaa      445
Lys Ser Asn Asp Ile Asn Val Tyr Gly Asp Val Val Met Asn His Lys         
                95                  100                 105             

tta gga gct gat ttc acg gag gca gtg caa gct gtt caa gta aat cct      493
Leu Gly Ala Asp Phe Thr Glu Ala Val Gln Ala Val Gln Val Asn Pro         
            110                 115                 120                 

tcg aac cgt tgg cag gat att tca ggt gtc tac acg att gat gca tgg      541
Ser Asn Arg Trp Gln Asp Ile Ser Gly Val Tyr Thr Ile Asp Ala Trp         
        125                 130                 135                     

acg gga ttt gac ttt cca ggg cgc aac aat gcc tat tcc gat ttt aaa      589
Thr Gly Phe Asp Phe Pro Gly Arg Asn Asn Ala Tyr Ser Asp Phe Lys         
    140                 145                 150                         

tgg aga tgg ttc cat ttt aat ggc gtt gac tgg gat caa cgc tat caa      637
Trp Arg Trp Phe His Phe Asn Gly Val Asp Trp Asp Gln Arg Tyr Gln         
155                 160                 165                 170         

gaa aac cat ctt ttt cgc ttt gca aat acg aac tgg aac tgg cga gtg      685
Glu Asn His Leu Phe Arg Phe Ala Asn Thr Asn Trp Asn Trp Arg Val         
                175                 180                 185             

gat gaa gag aat ggt aat tat gac tat tta tta gga tcg aac att gac      733
Asp Glu Glu Asn Gly Asn Tyr Asp Tyr Leu Leu Gly Ser Asn Ile Asp         
            190                 195                 200                 

ttt agc cac cca gag gtt caa gag gaa tta aag gat tgg ggg agc tgg      781
Phe Ser His Pro Glu Val Gln Glu Glu Leu Lys Asp Trp Gly Ser Trp         
        205                 210                 215                     

ttt acg gat gag cta gat tta gat ggg tat cga ttg gat gct att aag      829
Phe Thr Asp Glu Leu Asp Leu Asp Gly Tyr Arg Leu Asp Ala Ile Lys         
    220                 225                 230                         

cat att cca ttc tgg tat acg tca gat tgg gtt agg cat cag cga agt      877
His Ile Pro Phe Trp Tyr Thr Ser Asp Trp Val Arg His Gln Arg Ser         
235                 240                 245                 250         

gaa gca gac caa gat tta ttt gtc gta ggg gag tat tgg aag gat gac      925
Glu Ala Asp Gln Asp Leu Phe Val Val Gly Glu Tyr Trp Lys Asp Asp         
                255                 260                 265             

gta ggt gct ctc gaa ttt tat tta gat gaa atg aat tgg gag atg tct      973
Val Gly Ala Leu Glu Phe Tyr Leu Asp Glu Met Asn Trp Glu Met Ser         
            270                 275                 280                 

cta ttc gat gtt ccg ctc aat tat aat ttt tac cgg gct tca aag caa     1021
Leu Phe Asp Val Pro Leu Asn Tyr Asn Phe Tyr Arg Ala Ser Lys Gln         
        285                 290                 295                     

ggc gga agc tat gat atg cgt aat att tta cga gga tct tta gta gaa     1069
Gly Gly Ser Tyr Asp Met Arg Asn Ile Leu Arg Gly Ser Leu Val Glu         
    300                 305                 310                         

gca cat ccg att cat gca gtt acg ttt gtt gat aat cat gat act cag     1117
Ala His Pro Ile His Ala Val Thr Phe Val Asp Asn His Asp Thr Gln         
315                 320                 325                 330         

cca gga gag tca tta gaa tca tgg gtc gct gat tgg ttt aag cca ctt     1165
Pro Gly Glu Ser Leu Glu Ser Trp Val Ala Asp Trp Phe Lys Pro Leu         
                335                 340                 345             

gct tat gcg aca atc ttg acg cgt gaa ggt ggt tat cca aat gta ttt     1213
Ala Tyr Ala Thr Ile Leu Thr Arg Glu Gly Gly Tyr Pro Asn Val Phe         
            350                 355                 360                 

tac ggt gac tac tat ggg att cct aac gat aac att tca gct aag aag     1261
Tyr Gly Asp Tyr Tyr Gly Ile Pro Asn Asp Asn Ile Ser Ala Lys Lys         
        365                 370                 375                     

gat atg att gat gag ttg ctt gat gca cgt caa aat tac gca tat ggc     1309
Asp Met Ile Asp Glu Leu Leu Asp Ala Arg Gln Asn Tyr Ala Tyr Gly         
    380                 385                 390                         

aca caa cat gac tat ttt gat cat tgg gat atc gtt gga tgg aca aga     1357
Thr Gln His Asp Tyr Phe Asp His Trp Asp Ile Val Gly Trp Thr Arg         
395                 400                 405                 410         

gaa ggt aca tcc tca cgt cct aat tcg ggt ctt gct act att atg tcc     1405
Glu Gly Thr Ser Ser Arg Pro Asn Ser Gly Leu Ala Thr Ile Met Ser         
                415                 420                 425             

aat ggt cct gga gga tca aaa tgg atg tac gta gga cag caa cat gca     1453
Asn Gly Pro Gly Gly Ser Lys Trp Met Tyr Val Gly Gln Gln His Ala         
            430                 435                 440                 

gga caa acg tgg aca gat tta act ggc aat cac gcg gcg tcg gtt acg     1501
Gly Gln Thr Trp Thr Asp Leu Thr Gly Asn His Ala Ala Ser Val Thr         
        445                 450                 455                     

att aat ggt gat ggc tgg ggc gaa ttc ttt aca aat gga gga tct gta     1549
Ile Asn Gly Asp Gly Trp Gly Glu Phe Phe Thr Asn Gly Gly Ser Val         
    460                 465                 470                         

tcc gtg tat gtg aac caa taataaaaag ccttgagaag ggattcctcc            1597
Ser Val Tyr Val Asn Gln                                                 
475                 480                                                 

ctaactcaag gctttcttta tgtcgtttag ctcaacgctt ctacgaagct tta          1650


<210>  2

<211>  501

<212>  PRT

<213>  Bacillus sp.



<400>  2

Met Lys Arg Trp Val Val Ala Met Leu Ala Val Leu Phe Leu Phe Pro 
    -20                 -15                 -10                 


Ser Val Val Val Ala Asp Gly Leu Asn Gly Thr Met Met Gln Tyr Tyr 
-5              -1  1               5                   10      


Glu Trp His Leu Glu Asn Asp Gly Gln His Trp Asn Arg Leu His Asp 
            15                  20                  25          


Asp Ala Glu Ala Leu Ser Asn Ala Gly Ile Thr Ala Ile Trp Ile Pro 
        30                  35                  40              


Pro Ala Tyr Lys Gly Asn Ser Gln Ala Asp Val Gly Tyr Gly Ala Tyr 
    45                  50                  55                  


Asp Leu Tyr Asp Leu Gly Glu Phe Asn Gln Lys Gly Thr Val Arg Thr 
60                  65                  70                  75  


Lys Tyr Gly Thr Lys Ala Gln Leu Glu Arg Ala Ile Gly Ser Leu Lys 
                80                  85                  90      


Ser Asn Asp Ile Asn Val Tyr Gly Asp Val Val Met Asn His Lys Leu 
            95                  100                 105         


Gly Ala Asp Phe Thr Glu Ala Val Gln Ala Val Gln Val Asn Pro Ser 
        110                 115                 120             


Asn Arg Trp Gln Asp Ile Ser Gly Val Tyr Thr Ile Asp Ala Trp Thr 
    125                 130                 135                 


Gly Phe Asp Phe Pro Gly Arg Asn Asn Ala Tyr Ser Asp Phe Lys Trp 
140                 145                 150                 155 


Arg Trp Phe His Phe Asn Gly Val Asp Trp Asp Gln Arg Tyr Gln Glu 
                160                 165                 170     


Asn His Leu Phe Arg Phe Ala Asn Thr Asn Trp Asn Trp Arg Val Asp 
            175                 180                 185         


Glu Glu Asn Gly Asn Tyr Asp Tyr Leu Leu Gly Ser Asn Ile Asp Phe 
        190                 195                 200             


Ser His Pro Glu Val Gln Glu Glu Leu Lys Asp Trp Gly Ser Trp Phe 
    205                 210                 215                 


Thr Asp Glu Leu Asp Leu Asp Gly Tyr Arg Leu Asp Ala Ile Lys His 
220                 225                 230                 235 


Ile Pro Phe Trp Tyr Thr Ser Asp Trp Val Arg His Gln Arg Ser Glu 
                240                 245                 250     


Ala Asp Gln Asp Leu Phe Val Val Gly Glu Tyr Trp Lys Asp Asp Val 
            255                 260                 265         


Gly Ala Leu Glu Phe Tyr Leu Asp Glu Met Asn Trp Glu Met Ser Leu 
        270                 275                 280             


Phe Asp Val Pro Leu Asn Tyr Asn Phe Tyr Arg Ala Ser Lys Gln Gly 
    285                 290                 295                 


Gly Ser Tyr Asp Met Arg Asn Ile Leu Arg Gly Ser Leu Val Glu Ala 
300                 305                 310                 315 


His Pro Ile His Ala Val Thr Phe Val Asp Asn His Asp Thr Gln Pro 
                320                 325                 330     


Gly Glu Ser Leu Glu Ser Trp Val Ala Asp Trp Phe Lys Pro Leu Ala 
            335                 340                 345         


Tyr Ala Thr Ile Leu Thr Arg Glu Gly Gly Tyr Pro Asn Val Phe Tyr 
        350                 355                 360             


Gly Asp Tyr Tyr Gly Ile Pro Asn Asp Asn Ile Ser Ala Lys Lys Asp 
    365                 370                 375                 


Met Ile Asp Glu Leu Leu Asp Ala Arg Gln Asn Tyr Ala Tyr Gly Thr 
380                 385                 390                 395 


Gln His Asp Tyr Phe Asp His Trp Asp Ile Val Gly Trp Thr Arg Glu 
                400                 405                 410     


Gly Thr Ser Ser Arg Pro Asn Ser Gly Leu Ala Thr Ile Met Ser Asn 
            415                 420                 425         


Gly Pro Gly Gly Ser Lys Trp Met Tyr Val Gly Gln Gln His Ala Gly 
        430                 435                 440             


Gln Thr Trp Thr Asp Leu Thr Gly Asn His Ala Ala Ser Val Thr Ile 
    445                 450                 455                 


Asn Gly Asp Gly Trp Gly Glu Phe Phe Thr Asn Gly Gly Ser Val Ser 
460                 465                 470                 475 


Val Tyr Val Asn Gln 
                480 


<210>  3

<211>  1745

<212>  DNA

<213>  Bacillus



<220>

<221>  CDS

<222>  (190)..(1692)

<223>  



<220>

<221>  mat_peptide

<222>  (253)..()

<223>  



<220>

<221>  sig_peptide

<222>  (190)..(253)

<223>  



<400>  3
aactaagtaa catcgattca ggataaaagt atgcgaaacg atgcgcaaaa ctgcgcaact     60

actagcactc ttcagggact aaaccacctt ttttccaaaa atgacatcat ataaacaaat    120

ttgtctacca atcactattt aaagctgttt atgatatatg taagcgttat cattaaaagg    180

aggtatttg atg aga aga tgg gta gta gca atg ttg gca gtg tta ttt tta    231
          Met Arg Arg Trp Val Val Ala Met Leu Ala Val Leu Phe Leu       
              -20                 -15                 -10               

ttt cct tcg gta gta gtt gca gat gga ttg aac ggt acg atg atg cag      279
Phe Pro Ser Val Val Val Ala Asp Gly Leu Asn Gly Thr Met Met Gln         
        -5              -1  1               5                           

tat tat gag tgg cat ttg gaa aac gac ggg cag cat tgg aat cgg ttg      327
Tyr Tyr Glu Trp His Leu Glu Asn Asp Gly Gln His Trp Asn Arg Leu         
10                  15                  20                  25          

cac gat gat gcc gca gct ttg agt gat gct ggt att aca gct att tgg      375
His Asp Asp Ala Ala Ala Leu Ser Asp Ala Gly Ile Thr Ala Ile Trp         
                30                  35                  40              

att ccg cca gcc tac aaa ggt aat agt cag gcg gat gtt ggg tac ggt      423
Ile Pro Pro Ala Tyr Lys Gly Asn Ser Gln Ala Asp Val Gly Tyr Gly         
            45                  50                  55                  

gca tac gat ctt tat gat tta gga gag ttc aat caa aag ggt act gtt      471
Ala Tyr Asp Leu Tyr Asp Leu Gly Glu Phe Asn Gln Lys Gly Thr Val         
        60                  65                  70                      

cga acg aaa tac gga act aag gca cag ctt gaa cga gct att ggg tcc      519
Arg Thr Lys Tyr Gly Thr Lys Ala Gln Leu Glu Arg Ala Ile Gly Ser         
    75                  80                  85                          

ctt aaa tct aat gat atc aat gta tac gga gat gtc gtg atg aat cat      567
Leu Lys Ser Asn Asp Ile Asn Val Tyr Gly Asp Val Val Met Asn His         
90                  95                  100                 105         

aaa atg gga gct gat ttt acg gag gca gtg caa gct gtt caa gta aat      615
Lys Met Gly Ala Asp Phe Thr Glu Ala Val Gln Ala Val Gln Val Asn         
                110                 115                 120             

cca acg aat cgt tgg cag gat att tca ggt gcc tac acg att gat gcg      663
Pro Thr Asn Arg Trp Gln Asp Ile Ser Gly Ala Tyr Thr Ile Asp Ala         
            125                 130                 135                 

tgg acg ggt ttc gac ttt tca ggg cgt aac aac gcc tat tca gat ttt      711
Trp Thr Gly Phe Asp Phe Ser Gly Arg Asn Asn Ala Tyr Ser Asp Phe         
        140                 145                 150                     

aag tgg aga tgg ttc cat ttt aat ggt gtt gac tgg gat cag cgc tat      759
Lys Trp Arg Trp Phe His Phe Asn Gly Val Asp Trp Asp Gln Arg Tyr         
    155                 160                 165                         

caa gaa aat cat att ttc cgc ttt gca aat acg aac tgg aac tgg cga      807
Gln Glu Asn His Ile Phe Arg Phe Ala Asn Thr Asn Trp Asn Trp Arg         
170                 175                 180                 185         

gtg gat gaa gag aac ggt aat tat gat tac ctg tta gga tcg aat atc      855
Val Asp Glu Glu Asn Gly Asn Tyr Asp Tyr Leu Leu Gly Ser Asn Ile         
                190                 195                 200             

gac ttt agt cat cca gaa gta caa gat gag ttg aag gat tgg ggt agc      903
Asp Phe Ser His Pro Glu Val Gln Asp Glu Leu Lys Asp Trp Gly Ser         
            205                 210                 215                 

tgg ttt acc gat gag tta gat ttg gat ggt tat cgt tta gat gct att      951
Trp Phe Thr Asp Glu Leu Asp Leu Asp Gly Tyr Arg Leu Asp Ala Ile         
        220                 225                 230                     

aaa cat att cca ttc tgg tat aca tct gat tgg gtt cgg cat cag cgc      999
Lys His Ile Pro Phe Trp Tyr Thr Ser Asp Trp Val Arg His Gln Arg         
    235                 240                 245                         

aac gaa gca gat caa gat tta ttt gtc gta ggg gaa tat tgg aag gat     1047
Asn Glu Ala Asp Gln Asp Leu Phe Val Val Gly Glu Tyr Trp Lys Asp         
250                 255                 260                 265         

gac gta ggt gct ctc gaa ttt tat tta gat gaa atg aat tgg gag atg     1095
Asp Val Gly Ala Leu Glu Phe Tyr Leu Asp Glu Met Asn Trp Glu Met         
                270                 275                 280             

tct cta ttc gat gtt cca ctt aat tat aat ttt tac cgg gct tca caa     1143
Ser Leu Phe Asp Val Pro Leu Asn Tyr Asn Phe Tyr Arg Ala Ser Gln         
            285                 290                 295                 

caa ggt gga agc tat gat atg cgt aat att tta cga gga tct tta gta     1191
Gln Gly Gly Ser Tyr Asp Met Arg Asn Ile Leu Arg Gly Ser Leu Val         
        300                 305                 310                     

gaa gcg cat ccg atg cat gca gtt acg ttt gtt gat aat cat gat act     1239
Glu Ala His Pro Met His Ala Val Thr Phe Val Asp Asn His Asp Thr         
    315                 320                 325                         

cag cca ggg gag tca tta gag tca tgg gtt gct gat tgg ttt aag cca     1287
Gln Pro Gly Glu Ser Leu Glu Ser Trp Val Ala Asp Trp Phe Lys Pro         
330                 335                 340                 345         

ctt gct tat gcg aca att ttg acg cgt gaa ggt ggt tat cca aat gta     1335
Leu Ala Tyr Ala Thr Ile Leu Thr Arg Glu Gly Gly Tyr Pro Asn Val         
                350                 355                 360             

ttt tac ggt gat tac tat ggg att cct aac gat aac att tca gct aaa     1383
Phe Tyr Gly Asp Tyr Tyr Gly Ile Pro Asn Asp Asn Ile Ser Ala Lys         
            365                 370                 375                 

aaa gat atg att gat gag ctg ctt gat gca cgt caa aat tac gca tat     1431
Lys Asp Met Ile Asp Glu Leu Leu Asp Ala Arg Gln Asn Tyr Ala Tyr         
        380                 385                 390                     

ggc acg cag cat gac tat ttt gat cat tgg gat gtt gta gga tgg act     1479
Gly Thr Gln His Asp Tyr Phe Asp His Trp Asp Val Val Gly Trp Thr         
    395                 400                 405                         

agg gaa gga tct tcc tcc aga cct aat tca ggc ctt gcg act att atg     1527
Arg Glu Gly Ser Ser Ser Arg Pro Asn Ser Gly Leu Ala Thr Ile Met         
410                 415                 420                 425         

tcg aat gga cct ggt ggt tcc aag tgg atg tat gta gga cgt cag aat     1575
Ser Asn Gly Pro Gly Gly Ser Lys Trp Met Tyr Val Gly Arg Gln Asn         
                430                 435                 440             

gca gga caa aca tgg aca gat tta act ggt aat aac gga gcg tcc gtt     1623
Ala Gly Gln Thr Trp Thr Asp Leu Thr Gly Asn Asn Gly Ala Ser Val         
            445                 450                 455                 

aca att aat ggc gat gga tgg ggc gaa ttc ttt acg aat gga gga tct     1671
Thr Ile Asn Gly Asp Gly Trp Gly Glu Phe Phe Thr Asn Gly Gly Ser         
        460                 465                 470                     

gta tcc gtg tac gtg aac caa taacaaaaag ccttgagaag ggattcctcc        1722
Val Ser Val Tyr Val Asn Gln                                             
    475                 480                                             

ctaactcaag gctttcttta tgt                                           1745


<210>  4

<211>  501

<212>  PRT

<213>  Bacillus



<400>  4

Met Arg Arg Trp Val Val Ala Met Leu Ala Val Leu Phe Leu Phe Pro 
    -20                 -15                 -10                 


Ser Val Val Val Ala Asp Gly Leu Asn Gly Thr Met Met Gln Tyr Tyr 
-5              -1  1               5                   10      


Glu Trp His Leu Glu Asn Asp Gly Gln His Trp Asn Arg Leu His Asp 
            15                  20                  25          


Asp Ala Ala Ala Leu Ser Asp Ala Gly Ile Thr Ala Ile Trp Ile Pro 
        30                  35                  40              


Pro Ala Tyr Lys Gly Asn Ser Gln Ala Asp Val Gly Tyr Gly Ala Tyr 
    45                  50                  55                  


Asp Leu Tyr Asp Leu Gly Glu Phe Asn Gln Lys Gly Thr Val Arg Thr 
60                  65                  70                  75  


Lys Tyr Gly Thr Lys Ala Gln Leu Glu Arg Ala Ile Gly Ser Leu Lys 
                80                  85                  90      


Ser Asn Asp Ile Asn Val Tyr Gly Asp Val Val Met Asn His Lys Met 
            95                  100                 105         


Gly Ala Asp Phe Thr Glu Ala Val Gln Ala Val Gln Val Asn Pro Thr 
        110                 115                 120             


Asn Arg Trp Gln Asp Ile Ser Gly Ala Tyr Thr Ile Asp Ala Trp Thr 
    125                 130                 135                 


Gly Phe Asp Phe Ser Gly Arg Asn Asn Ala Tyr Ser Asp Phe Lys Trp 
140                 145                 150                 155 


Arg Trp Phe His Phe Asn Gly Val Asp Trp Asp Gln Arg Tyr Gln Glu 
                160                 165                 170     


Asn His Ile Phe Arg Phe Ala Asn Thr Asn Trp Asn Trp Arg Val Asp 
            175                 180                 185         


Glu Glu Asn Gly Asn Tyr Asp Tyr Leu Leu Gly Ser Asn Ile Asp Phe 
        190                 195                 200             


Ser His Pro Glu Val Gln Asp Glu Leu Lys Asp Trp Gly Ser Trp Phe 
    205                 210                 215                 


Thr Asp Glu Leu Asp Leu Asp Gly Tyr Arg Leu Asp Ala Ile Lys His 
220                 225                 230                 235 


Ile Pro Phe Trp Tyr Thr Ser Asp Trp Val Arg His Gln Arg Asn Glu 
                240                 245                 250     


Ala Asp Gln Asp Leu Phe Val Val Gly Glu Tyr Trp Lys Asp Asp Val 
            255                 260                 265         


Gly Ala Leu Glu Phe Tyr Leu Asp Glu Met Asn Trp Glu Met Ser Leu 
        270                 275                 280             


Phe Asp Val Pro Leu Asn Tyr Asn Phe Tyr Arg Ala Ser Gln Gln Gly 
    285                 290                 295                 


Gly Ser Tyr Asp Met Arg Asn Ile Leu Arg Gly Ser Leu Val Glu Ala 
300                 305                 310                 315 


His Pro Met His Ala Val Thr Phe Val Asp Asn His Asp Thr Gln Pro 
                320                 325                 330     


Gly Glu Ser Leu Glu Ser Trp Val Ala Asp Trp Phe Lys Pro Leu Ala 
            335                 340                 345         


Tyr Ala Thr Ile Leu Thr Arg Glu Gly Gly Tyr Pro Asn Val Phe Tyr 
        350                 355                 360             


Gly Asp Tyr Tyr Gly Ile Pro Asn Asp Asn Ile Ser Ala Lys Lys Asp 
    365                 370                 375                 


Met Ile Asp Glu Leu Leu Asp Ala Arg Gln Asn Tyr Ala Tyr Gly Thr 
380                 385                 390                 395 


Gln His Asp Tyr Phe Asp His Trp Asp Val Val Gly Trp Thr Arg Glu 
                400                 405                 410     


Gly Ser Ser Ser Arg Pro Asn Ser Gly Leu Ala Thr Ile Met Ser Asn 
            415                 420                 425         


Gly Pro Gly Gly Ser Lys Trp Met Tyr Val Gly Arg Gln Asn Ala Gly 
        430                 435                 440             


Gln Thr Trp Thr Asp Leu Thr Gly Asn Asn Gly Ala Ser Val Thr Ile 
    445                 450                 455                 


Asn Gly Asp Gly Trp Gly Glu Phe Phe Thr Asn Gly Gly Ser Val Ser 
460                 465                 470                 475 


Val Tyr Val Asn Gln 
                480 


<210>  5

<211>  1920

<212>  DNA

<213>  Bacillus licheniformis



<220>

<221>  CDS

<222>  (421)..(1872)

<223>  



<400>  5
cggaagattg gaagtacaaa aataagcaaa agattgtcaa tcatgtcatg agccatgcgg     60

gagacggaaa aatcgtctta atgcacgata tttatgcaac gttcgcagat gctgctgaag    120

agattattaa aaagctgaaa gcaaaaggct atcaattggt aactgtatct cagcttgaag    180

aagtgaagaa gcagagaggc tattgaataa atgagtagaa gcgccatatc ggcgcttttc    240

ttttggaaga aaatataggg aaaatggtac ttgttaaaaa ttcggaatat ttatacaaca    300

tcatatgttt cacattgaaa ggggaggaga atcatgaaac aacaaaaacg gctttacgcc    360

cgattgctga cgctgttatt tgcgctcatc ttcttgctgc ctcattctgc agcagcggcg    420

gca aat ctt aat ggg acg ctg atg cag tat ttt gaa tgg tac atg ccc      468
Ala Asn Leu Asn Gly Thr Leu Met Gln Tyr Phe Glu Trp Tyr Met Pro         
1               5                   10                  15              

aat gac ggc caa cat tgg agg cgt ttg caa aac gac tcg gca tat ttg      516
Asn Asp Gly Gln His Trp Arg Arg Leu Gln Asn Asp Ser Ala Tyr Leu         
            20                  25                  30                  

gct gaa cac ggt att act gcc gtc tgg att ccc ccg gca tat aag gga      564
Ala Glu His Gly Ile Thr Ala Val Trp Ile Pro Pro Ala Tyr Lys Gly         
        35                  40                  45                      

acg agc caa gcg gat gtg ggc tac ggt gct tac gac ctt tat gat tta      612
Thr Ser Gln Ala Asp Val Gly Tyr Gly Ala Tyr Asp Leu Tyr Asp Leu         
    50                  55                  60                          

ggg gag ttt cat caa aaa ggg acg gtt cgg aca aag tac ggc aca aaa      660
Gly Glu Phe His Gln Lys Gly Thr Val Arg Thr Lys Tyr Gly Thr Lys         
65                  70                  75                  80          

gga gag ctg caa tct gcg atc aaa agt ctt cat tcc cgc gac att aac      708
Gly Glu Leu Gln Ser Ala Ile Lys Ser Leu His Ser Arg Asp Ile Asn         
                85                  90                  95              

gtt tac ggg gat gtg gtc atc aac cac aaa ggc ggc gct gat gcg acc      756
Val Tyr Gly Asp Val Val Ile Asn His Lys Gly Gly Ala Asp Ala Thr         
            100                 105                 110                 

gaa gat gta acc gcg gtt gaa gtc gat ccc gct gac cgc aac cgc gta      804
Glu Asp Val Thr Ala Val Glu Val Asp Pro Ala Asp Arg Asn Arg Val         
        115                 120                 125                     

att tca gga gaa cac cta att aaa gcc tgg aca cat ttt cat ttt ccg      852
Ile Ser Gly Glu His Leu Ile Lys Ala Trp Thr His Phe His Phe Pro         
    130                 135                 140                         

ggg cgc ggc agc aca tac agc gat ttt aaa tgg cat tgg tac cat ttt      900
Gly Arg Gly Ser Thr Tyr Ser Asp Phe Lys Trp His Trp Tyr His Phe         
145                 150                 155                 160         

gac gga acc gat tgg gac gag tcc cga aag ctg aac cgc atc tat aag      948
Asp Gly Thr Asp Trp Asp Glu Ser Arg Lys Leu Asn Arg Ile Tyr Lys         
                165                 170                 175             

ttt caa gga aag gct tgg gat tgg gaa gtt tcc aat gaa aac ggc aac      996
Phe Gln Gly Lys Ala Trp Asp Trp Glu Val Ser Asn Glu Asn Gly Asn         
            180                 185                 190                 

tat gat tat ttg atg tat gcc gac atc gat tat gac cat cct gat gtc     1044
Tyr Asp Tyr Leu Met Tyr Ala Asp Ile Asp Tyr Asp His Pro Asp Val         
        195                 200                 205                     

gca gca gaa att aag aga tgg ggc act tgg tat gcc aat gaa ctg caa     1092
Ala Ala Glu Ile Lys Arg Trp Gly Thr Trp Tyr Ala Asn Glu Leu Gln         
    210                 215                 220                         

ttg gac ggt ttc cgt ctt gat gct gtc aaa cac att aaa ttt tct ttt     1140
Leu Asp Gly Phe Arg Leu Asp Ala Val Lys His Ile Lys Phe Ser Phe         
225                 230                 235                 240         

ttg cgg gat tgg gtt aat cat gtc agg gaa aaa acg ggg aag gaa atg     1188
Leu Arg Asp Trp Val Asn His Val Arg Glu Lys Thr Gly Lys Glu Met         
                245                 250                 255             

ttt acg gta gct gaa tat tgg cag aat gac ttg ggc gcg ctg gaa aac     1236
Phe Thr Val Ala Glu Tyr Trp Gln Asn Asp Leu Gly Ala Leu Glu Asn         
            260                 265                 270                 

tat ttg aac aaa aca aat ttt aat cat tca gtg ttt gac gtg ccg ctt     1284
Tyr Leu Asn Lys Thr Asn Phe Asn His Ser Val Phe Asp Val Pro Leu         
        275                 280                 285                     

cat tat cag ttc cat gct gca tcg aca cag gga ggc ggc tat gat atg     1332
His Tyr Gln Phe His Ala Ala Ser Thr Gln Gly Gly Gly Tyr Asp Met         
    290                 295                 300                         

agg aaa ttg ctg aac ggt acg gtc gtt tcc aag cat ccg ttg aaa tcg     1380
Arg Lys Leu Leu Asn Gly Thr Val Val Ser Lys His Pro Leu Lys Ser         
305                 310                 315                 320         

gtt aca ttt gtc gat aac cat gat aca cag ccg ggg caa tcg ctt gag     1428
Val Thr Phe Val Asp Asn His Asp Thr Gln Pro Gly Gln Ser Leu Glu         
                325                 330                 335             

tcg act gtc caa aca tgg ttt aag ccg ctt gct tac gct ttt att ctc     1476
Ser Thr Val Gln Thr Trp Phe Lys Pro Leu Ala Tyr Ala Phe Ile Leu         
            340                 345                 350                 

aca agg gaa tct gga tac cct cag gtt ttc tac ggg gat atg tac ggg     1524
Thr Arg Glu Ser Gly Tyr Pro Gln Val Phe Tyr Gly Asp Met Tyr Gly         
        355                 360                 365                     

acg aaa gga gac tcc cag cgc gaa att cct gcc ttg aaa cac aaa att     1572
Thr Lys Gly Asp Ser Gln Arg Glu Ile Pro Ala Leu Lys His Lys Ile         
    370                 375                 380                         

gaa ccg atc tta aaa gcg aga aaa cag tat gcg tac gga gca cag cat     1620
Glu Pro Ile Leu Lys Ala Arg Lys Gln Tyr Ala Tyr Gly Ala Gln His         
385                 390                 395                 400         

gat tat ttc gac cac cat gac att gtc ggc tgg aca agg gaa ggc gac     1668
Asp Tyr Phe Asp His His Asp Ile Val Gly Trp Thr Arg Glu Gly Asp         
                405                 410                 415             

agc tcg gtt gca aat tca ggt ttg gcg gca tta ata aca gac gga ccc     1716
Ser Ser Val Ala Asn Ser Gly Leu Ala Ala Leu Ile Thr Asp Gly Pro         
            420                 425                 430                 

ggt ggg gca aag cga atg tat gtc ggc cgg caa aac gcc ggt gag aca     1764
Gly Gly Ala Lys Arg Met Tyr Val Gly Arg Gln Asn Ala Gly Glu Thr         
        435                 440                 445                     

tgg cat gac att acc gga aac cgt tcg gag ccg gtt gtc atc aat tcg     1812
Trp His Asp Ile Thr Gly Asn Arg Ser Glu Pro Val Val Ile Asn Ser         
    450                 455                 460                         

gaa ggc tgg gga gag ttt cac gta aac ggc ggg tcg gtt tca att tat     1860
Glu Gly Trp Gly Glu Phe His Val Asn Gly Gly Ser Val Ser Ile Tyr         
465                 470                 475                 480         

gtt caa aga tag aagagcagag aggacggatt tcctgaagga aatccgtttt         1912
Val Gln Arg                                                             
                                                                        

tttatttt                                                            1920


<210>  6

<211>  483

<212>  PRT

<213>  Bacillus licheniformis



<400>  6

Ala Asn Leu Asn Gly Thr Leu Met Gln Tyr Phe Glu Trp Tyr Met Pro 
1               5                   10                  15      


Asn Asp Gly Gln His Trp Arg Arg Leu Gln Asn Asp Ser Ala Tyr Leu 
            20                  25                  30          


Ala Glu His Gly Ile Thr Ala Val Trp Ile Pro Pro Ala Tyr Lys Gly 
        35                  40                  45              


Thr Ser Gln Ala Asp Val Gly Tyr Gly Ala Tyr Asp Leu Tyr Asp Leu 
    50                  55                  60                  


Gly Glu Phe His Gln Lys Gly Thr Val Arg Thr Lys Tyr Gly Thr Lys 
65                  70                  75                  80  


Gly Glu Leu Gln Ser Ala Ile Lys Ser Leu His Ser Arg Asp Ile Asn 
                85                  90                  95      


Val Tyr Gly Asp Val Val Ile Asn His Lys Gly Gly Ala Asp Ala Thr 
            100                 105                 110         


Glu Asp Val Thr Ala Val Glu Val Asp Pro Ala Asp Arg Asn Arg Val 
        115                 120                 125             


Ile Ser Gly Glu His Leu Ile Lys Ala Trp Thr His Phe His Phe Pro 
    130                 135                 140                 


Gly Arg Gly Ser Thr Tyr Ser Asp Phe Lys Trp His Trp Tyr His Phe 
145                 150                 155                 160 


Asp Gly Thr Asp Trp Asp Glu Ser Arg Lys Leu Asn Arg Ile Tyr Lys 
                165                 170                 175     


Phe Gln Gly Lys Ala Trp Asp Trp Glu Val Ser Asn Glu Asn Gly Asn 
            180                 185                 190         


Tyr Asp Tyr Leu Met Tyr Ala Asp Ile Asp Tyr Asp His Pro Asp Val 
        195                 200                 205             


Ala Ala Glu Ile Lys Arg Trp Gly Thr Trp Tyr Ala Asn Glu Leu Gln 
    210                 215                 220                 


Leu Asp Gly Phe Arg Leu Asp Ala Val Lys His Ile Lys Phe Ser Phe 
225                 230                 235                 240 


Leu Arg Asp Trp Val Asn His Val Arg Glu Lys Thr Gly Lys Glu Met 
                245                 250                 255     


Phe Thr Val Ala Glu Tyr Trp Gln Asn Asp Leu Gly Ala Leu Glu Asn 
            260                 265                 270         


Tyr Leu Asn Lys Thr Asn Phe Asn His Ser Val Phe Asp Val Pro Leu 
        275                 280                 285             


His Tyr Gln Phe His Ala Ala Ser Thr Gln Gly Gly Gly Tyr Asp Met 
    290                 295                 300                 


Arg Lys Leu Leu Asn Gly Thr Val Val Ser Lys His Pro Leu Lys Ser 
305                 310                 315                 320 


Val Thr Phe Val Asp Asn His Asp Thr Gln Pro Gly Gln Ser Leu Glu 
                325                 330                 335     


Ser Thr Val Gln Thr Trp Phe Lys Pro Leu Ala Tyr Ala Phe Ile Leu 
            340                 345                 350         


Thr Arg Glu Ser Gly Tyr Pro Gln Val Phe Tyr Gly Asp Met Tyr Gly 
        355                 360                 365             


Thr Lys Gly Asp Ser Gln Arg Glu Ile Pro Ala Leu Lys His Lys Ile 
    370                 375                 380                 


Glu Pro Ile Leu Lys Ala Arg Lys Gln Tyr Ala Tyr Gly Ala Gln His 
385                 390                 395                 400 


Asp Tyr Phe Asp His His Asp Ile Val Gly Trp Thr Arg Glu Gly Asp 
                405                 410                 415     


Ser Ser Val Ala Asn Ser Gly Leu Ala Ala Leu Ile Thr Asp Gly Pro 
            420                 425                 430         


Gly Gly Ala Lys Arg Met Tyr Val Gly Arg Gln Asn Ala Gly Glu Thr 
        435                 440                 445             


Trp His Asp Ile Thr Gly Asn Arg Ser Glu Pro Val Val Ile Asn Ser 
    450                 455                 460                 


Glu Gly Trp Gly Glu Phe His Val Asn Gly Gly Ser Val Ser Ile Tyr 
465                 470                 475                 480 


Val Gln Arg 
            


<210>  7

<211>  1776

<212>  DNA

<213>  Bacillus sp.



<220>

<221>  CDS

<222>  (145)..(1692)

<223>  



<220>

<221>  mat_peptide

<222>  (238)..()

<223>  



<220>

<221>  sig_peptide

<222>  (145)..(238)

<223>  



<400>  7
atataaattt gaaatgaaca cctatgaaaa tatggtagcg attgcgcgac gagaaaaaac     60

ttgggagtta ggaagtgata ttaaaggatt ttttttgact tgttgtgaaa acgcttgcat    120

aaattgaagg agagggtgct tttt atg aaa ctt cat aac cgt ata att agc       171
                           Met Lys Leu His Asn Arg Ile Ile Ser          
                               -30                 -25                  

gta cta tta aca cta ttg tta gct gta gct gtt ttg ttt cca tat atg      219
Val Leu Leu Thr Leu Leu Leu Ala Val Ala Val Leu Phe Pro Tyr Met         
        -20                 -15                 -10                     

acg gaa cca gca caa gcc cat cat aat ggg acg aat ggg acc atg atg      267
Thr Glu Pro Ala Gln Ala His His Asn Gly Thr Asn Gly Thr Met Met         
    -5              -1  1               5                   10          

cag tat ttt gaa tgg cat ttg cca aat gac ggg aac cac tgg aac agg      315
Gln Tyr Phe Glu Trp His Leu Pro Asn Asp Gly Asn His Trp Asn Arg         
                15                  20                  25              

tta cga gat gac gca gct aac tta aag agt aaa ggg att acc gct gtt      363
Leu Arg Asp Asp Ala Ala Asn Leu Lys Ser Lys Gly Ile Thr Ala Val         
            30                  35                  40                  

tgg att cct cct gca tgg aag ggg act tcg caa aat gat gtt ggg tat      411
Trp Ile Pro Pro Ala Trp Lys Gly Thr Ser Gln Asn Asp Val Gly Tyr         
        45                  50                  55                      

ggt gcc tat gat ttg tac gat ctt ggt gag ttt aac caa aag gga acc      459
Gly Ala Tyr Asp Leu Tyr Asp Leu Gly Glu Phe Asn Gln Lys Gly Thr         
    60                  65                  70                          

gtc cgt aca aaa tat ggc aca agg agt cag ttg caa ggt gcc gtg aca      507
Val Arg Thr Lys Tyr Gly Thr Arg Ser Gln Leu Gln Gly Ala Val Thr         
75                  80                  85                  90          

tct ttg aaa aat aac ggg att caa gtt tat ggg gat gtc gtg atg aat      555
Ser Leu Lys Asn Asn Gly Ile Gln Val Tyr Gly Asp Val Val Met Asn         
                95                  100                 105             

cat aaa ggt gga gca gac ggg aca gag atg gta aat gcg gtg gaa gtg      603
His Lys Gly Gly Ala Asp Gly Thr Glu Met Val Asn Ala Val Glu Val         
            110                 115                 120                 

aac cga agc aac cga aac caa gaa ata tca ggt gaa tac acc att gaa      651
Asn Arg Ser Asn Arg Asn Gln Glu Ile Ser Gly Glu Tyr Thr Ile Glu         
        125                 130                 135                     

gca tgg acg aaa ttt gat ttc cct gga aga gga aat acc cat tcc aac      699
Ala Trp Thr Lys Phe Asp Phe Pro Gly Arg Gly Asn Thr His Ser Asn         
    140                 145                 150                         

ttt aaa tgg cgc tgg tat cat ttt gat ggg aca gat tgg gat cag tca      747
Phe Lys Trp Arg Trp Tyr His Phe Asp Gly Thr Asp Trp Asp Gln Ser         
155                 160                 165                 170         

cgt cag ctt cag aac aaa ata tat aaa ttc aga ggt acc gga aag gca      795
Arg Gln Leu Gln Asn Lys Ile Tyr Lys Phe Arg Gly Thr Gly Lys Ala         
                175                 180                 185             

tgg gac tgg gaa gta gat ata gag aac ggc aac tat gat tac ctt atg      843
Trp Asp Trp Glu Val Asp Ile Glu Asn Gly Asn Tyr Asp Tyr Leu Met         
            190                 195                 200                 

tat gca gac att gat atg gat cat cca gaa gta atc aat gaa ctt aga      891
Tyr Ala Asp Ile Asp Met Asp His Pro Glu Val Ile Asn Glu Leu Arg         
        205                 210                 215                     

aat tgg gga gtt tgg tat aca aat aca ctt aat cta gat gga ttt aga      939
Asn Trp Gly Val Trp Tyr Thr Asn Thr Leu Asn Leu Asp Gly Phe Arg         
    220                 225                 230                         

atc gat gct gtg aaa cat att aaa tac agc tat acg aga gat tgg cta      987
Ile Asp Ala Val Lys His Ile Lys Tyr Ser Tyr Thr Arg Asp Trp Leu         
235                 240                 245                 250         

aca cat gtg cgt aac acc aca ggt aaa cca atg ttt gca gtt gca gaa     1035
Thr His Val Arg Asn Thr Thr Gly Lys Pro Met Phe Ala Val Ala Glu         
                255                 260                 265             

ttt tgg aaa aat gac ctt gct gca atc gaa aac tat tta aat aaa aca     1083
Phe Trp Lys Asn Asp Leu Ala Ala Ile Glu Asn Tyr Leu Asn Lys Thr         
            270                 275                 280                 

agt tgg aat cac tcc gtg ttc gat gtt cct ctt cat tat aat ttg tac     1131
Ser Trp Asn His Ser Val Phe Asp Val Pro Leu His Tyr Asn Leu Tyr         
        285                 290                 295                     

aat gca tct aat agt ggt ggc tat ttt gat atg aga aat att tta aat     1179
Asn Ala Ser Asn Ser Gly Gly Tyr Phe Asp Met Arg Asn Ile Leu Asn         
    300                 305                 310                         

ggt tct gtc gta caa aaa cac cct ata cat gca gtc aca ttt gtt gat     1227
Gly Ser Val Val Gln Lys His Pro Ile His Ala Val Thr Phe Val Asp         
315                 320                 325                 330         

aac cat gac tct cag cca gga gaa gca ttg gaa tcc ttt gtt caa tcg     1275
Asn His Asp Ser Gln Pro Gly Glu Ala Leu Glu Ser Phe Val Gln Ser         
                335                 340                 345             

tgg ttc aaa cca ctg gca tat gca ttg att ctg aca agg gag caa ggt     1323
Trp Phe Lys Pro Leu Ala Tyr Ala Leu Ile Leu Thr Arg Glu Gln Gly         
            350                 355                 360                 

tac cct tcc gta ttt tac ggt gat tac tac ggt ata cca act cat ggt     1371
Tyr Pro Ser Val Phe Tyr Gly Asp Tyr Tyr Gly Ile Pro Thr His Gly         
        365                 370                 375                     

gtt cct tcg atg aaa tct aaa att gat cca ctt ctg cag gca cgt caa     1419
Val Pro Ser Met Lys Ser Lys Ile Asp Pro Leu Leu Gln Ala Arg Gln         
    380                 385                 390                         

acg tat gcc tac gga acc caa cat gat tat ttt gat cat cat gat att     1467
Thr Tyr Ala Tyr Gly Thr Gln His Asp Tyr Phe Asp His His Asp Ile         
395                 400                 405                 410         

atc ggc tgg acg aga gaa ggg gac agc tcc cac cca aat tca gga ctt     1515
Ile Gly Trp Thr Arg Glu Gly Asp Ser Ser His Pro Asn Ser Gly Leu         
                415                 420                 425             

gca act att atg tcc gat ggg cca ggg ggt aat aaa tgg atg tat gtc     1563
Ala Thr Ile Met Ser Asp Gly Pro Gly Gly Asn Lys Trp Met Tyr Val         
            430                 435                 440                 

ggg aaa cat aaa gct ggc caa gta tgg aga gat atc acc gga aat agg     1611
Gly Lys His Lys Ala Gly Gln Val Trp Arg Asp Ile Thr Gly Asn Arg         
        445                 450                 455                     

tct ggt acc gtc acc att aat gca gat ggt tgg ggg aat ttc act gta     1659
Ser Gly Thr Val Thr Ile Asn Ala Asp Gly Trp Gly Asn Phe Thr Val         
    460                 465                 470                         

aac gga ggg gca gtt tcg gtt tgg gtg aag caa taaataagga acaagaggcg   1712
Asn Gly Gly Ala Val Ser Val Trp Val Lys Gln                             
475                 480                 485                             

aaaattactt tcctacatgc agagctttcc gatcactcat acacccaata taaattggaa   1772

gctt                                                                1776


<210>  8

<211>  516

<212>  PRT

<213>  Bacillus sp.



<400>  8

Met Lys Leu His Asn Arg Ile Ile Ser Val Leu Leu Thr Leu Leu Leu 
    -30                 -25                 -20                 


Ala Val Ala Val Leu Phe Pro Tyr Met Thr Glu Pro Ala Gln Ala His 
-15                 -10                 -5              -1  1   


His Asn Gly Thr Asn Gly Thr Met Met Gln Tyr Phe Glu Trp His Leu 
            5                   10                  15          


Pro Asn Asp Gly Asn His Trp Asn Arg Leu Arg Asp Asp Ala Ala Asn 
        20                  25                  30              


Leu Lys Ser Lys Gly Ile Thr Ala Val Trp Ile Pro Pro Ala Trp Lys 
    35                  40                  45                  


Gly Thr Ser Gln Asn Asp Val Gly Tyr Gly Ala Tyr Asp Leu Tyr Asp 
50                  55                  60                  65  


Leu Gly Glu Phe Asn Gln Lys Gly Thr Val Arg Thr Lys Tyr Gly Thr 
                70                  75                  80      


Arg Ser Gln Leu Gln Gly Ala Val Thr Ser Leu Lys Asn Asn Gly Ile 
            85                  90                  95          


Gln Val Tyr Gly Asp Val Val Met Asn His Lys Gly Gly Ala Asp Gly 
        100                 105                 110             


Thr Glu Met Val Asn Ala Val Glu Val Asn Arg Ser Asn Arg Asn Gln 
    115                 120                 125                 


Glu Ile Ser Gly Glu Tyr Thr Ile Glu Ala Trp Thr Lys Phe Asp Phe 
130                 135                 140                 145 


Pro Gly Arg Gly Asn Thr His Ser Asn Phe Lys Trp Arg Trp Tyr His 
                150                 155                 160     


Phe Asp Gly Thr Asp Trp Asp Gln Ser Arg Gln Leu Gln Asn Lys Ile 
            165                 170                 175         


Tyr Lys Phe Arg Gly Thr Gly Lys Ala Trp Asp Trp Glu Val Asp Ile 
        180                 185                 190             


Glu Asn Gly Asn Tyr Asp Tyr Leu Met Tyr Ala Asp Ile Asp Met Asp 
    195                 200                 205                 


His Pro Glu Val Ile Asn Glu Leu Arg Asn Trp Gly Val Trp Tyr Thr 
210                 215                 220                 225 


Asn Thr Leu Asn Leu Asp Gly Phe Arg Ile Asp Ala Val Lys His Ile 
                230                 235                 240     


Lys Tyr Ser Tyr Thr Arg Asp Trp Leu Thr His Val Arg Asn Thr Thr 
            245                 250                 255         


Gly Lys Pro Met Phe Ala Val Ala Glu Phe Trp Lys Asn Asp Leu Ala 
        260                 265                 270             


Ala Ile Glu Asn Tyr Leu Asn Lys Thr Ser Trp Asn His Ser Val Phe 
    275                 280                 285                 


Asp Val Pro Leu His Tyr Asn Leu Tyr Asn Ala Ser Asn Ser Gly Gly 
290                 295                 300                 305 


Tyr Phe Asp Met Arg Asn Ile Leu Asn Gly Ser Val Val Gln Lys His 
                310                 315                 320     


Pro Ile His Ala Val Thr Phe Val Asp Asn His Asp Ser Gln Pro Gly 
            325                 330                 335         


Glu Ala Leu Glu Ser Phe Val Gln Ser Trp Phe Lys Pro Leu Ala Tyr 
        340                 345                 350             


Ala Leu Ile Leu Thr Arg Glu Gln Gly Tyr Pro Ser Val Phe Tyr Gly 
    355                 360                 365                 


Asp Tyr Tyr Gly Ile Pro Thr His Gly Val Pro Ser Met Lys Ser Lys 
370                 375                 380                 385 


Ile Asp Pro Leu Leu Gln Ala Arg Gln Thr Tyr Ala Tyr Gly Thr Gln 
                390                 395                 400     


His Asp Tyr Phe Asp His His Asp Ile Ile Gly Trp Thr Arg Glu Gly 
            405                 410                 415         


Asp Ser Ser His Pro Asn Ser Gly Leu Ala Thr Ile Met Ser Asp Gly 
        420                 425                 430             


Pro Gly Gly Asn Lys Trp Met Tyr Val Gly Lys His Lys Ala Gly Gln 
    435                 440                 445                 


Val Trp Arg Asp Ile Thr Gly Asn Arg Ser Gly Thr Val Thr Ile Asn 
450                 455                 460                 465 


Ala Asp Gly Trp Gly Asn Phe Thr Val Asn Gly Gly Ala Val Ser Val 
                470                 475                 480     


Trp Val Lys Gln 
            485 


<210>  9

<211>  40

<212>  DNA

<213>  Artificial sequence



<220>

<223>  Primer DAX-8N

<400>  9
gctgcggccg ctgcagatgg mttgaayggw acgatgatgc                           40


<210>  10

<211>  43

<212>  DNA

<213>  Artificial sequence



<220>

<223>  Primer DAX-8C

<400>  10
ggccgtcgac ttattggttc acrtacacgg atacagatcc tcc                       43


<210>  11

<211>  32

<212>  DNA

<213>  Artificial sequence



<220>

<223>  Primer Amrk752-E84Q

<400>  11
ctaaggcaca gcttcaacga gctattgggt cc                                   32


<210>  12

<211>  35

<212>  DNA

<213>  Artificial sequence



<220>

<223>  Primer Amrk752-N96D

<400>  12
ccttaaatct aatgatatcg atgtatacgg agatg                                35


<210>  13

<211>  34

<212>  DNA

<213>  Artificial sequence



<220>

<223>  Primer Amrk752-A315S

<400>  13
ttataatttt taccggtctt cacaacaagg tgga                                 34


<210>  14

<211>  32

<212>  DNA

<213>  Artificial sequence



<220>

<223>  Primer Amrk752-A445V

<400>  14
gtaggacgtc agaatgtagg acaaacatgg ac                                   32


<210>  15

<211>  35

<212>  DNA

<213>  Artificial sequence



<220>

<223>  Primer Amrk752-G464N

<400>  15
ccgttacaat taataacgat ggatggggcg aattc                                35


<210>  16

<211>  34

<212>  DNA

<213>  Artificial sequence



<220>

<223>  Primer Amrk230-N121D

<400>  16
gcaagctgtt caagtagatc caacgaatcg ttgg                                 34


<210>  17

<211>  34

<212>  DNA

<213>  Artificial sequence



<220>

<223>  Primer Amrk230-N390H

<400>  17
gcttgatgca cgtcaagatt acgcatatgg cacg                                 34


